Purification and characterization of type I DNA topoisomerase from Lentinus edodes
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Purification and characterization of a type I DNA topoisomerase from calf thymus mitochondria.
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* To whom correspondence should be addrcsscd. Step 2・ . To 61 of homogenate, DEAE-cel]ulose (600g, wet wci.oht) pre-equilibruted with 10mM sodium acctate buffer (pH 6,O) was added and thc suspension was stirred gent]y at O"C fbr 3emin, The DEAE-cellu]ose-udsorbcd protein was coilected by suction and washed with 3 1 of thc same bufi'er. then suspendcd in the same buffcr. The column packcd -・ith ...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1986
ISSN: 0378-1097
DOI: 10.1016/0378-1097(86)90398-8